欧美边做饭边被躁bd在线看,欧美激情一区二区三区,亚洲熟妇av一区二区三区宅男,国产av在线观看

咨詢熱線

15000266580

當(dāng)前位置:首頁(yè) >產(chǎn)品中心>細(xì)胞庫(kù)>小鼠腫瘤細(xì)胞、癌細(xì)胞>CCL-1.3 L-M(TK-小鼠胸腺激酶缺陷細(xì)胞株

CCL-1.3 L-M(TK-小鼠胸腺激酶缺陷細(xì)胞株

簡(jiǎn)要描述:CCL-1.3 L-M(TK-) 小鼠胸腺激酶缺陷細(xì)胞株,
CCL-1.3 L-M(TK-小鼠胸腺激酶缺陷細(xì)胞株
ATCC 細(xì)胞|細(xì)胞系|細(xì)胞株|腫瘤細(xì)胞|細(xì)胞|貼壁細(xì)胞|懸浮細(xì)胞|,
細(xì)胞庫(kù)管理規(guī)范,
提供的細(xì)胞株背景清楚,提供參考文獻(xiàn)和*培養(yǎng)條件

  • 產(chǎn)品型號(hào):
  • 廠商性質(zhì):生產(chǎn)廠家
  • 更新時(shí)間:2025-04-27
  • 訪  問(wèn)  量:2517

產(chǎn)品分類

Product Category

詳細(xì)介紹

CCL-1.3 L-M(TK-) 小鼠胸腺激酶缺陷細(xì)胞株,ATCC 細(xì)胞|細(xì)胞系|細(xì)胞株|腫瘤細(xì)胞|細(xì)胞|貼壁細(xì)胞|懸浮細(xì)胞|,細(xì)胞庫(kù)管理規(guī)范,提供的細(xì)胞株背景清楚,提供參考文獻(xiàn)和i優(yōu)培養(yǎng)條件


CCL-1.3 L-M(TK-) 小鼠胸腺激酶缺陷細(xì)胞株

ATCC® Number: CCL-1.3™    Price: $329.00
Designations: L-M(TK-) [LM(tk-), LMTK-]

Depositors: TR Chen

Biosafety Level:1

Shipped: frozen

Medium & Serum: See Propagation

Growth Properties:adherent

Organism:Mus musculus (mouse)

Morphology:fibroblast


Source:Tissue: subcutaneous connective tissue; areolar and adipose
Strain: C3H/An


Permits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimay responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
CCL-1.3 L-M(TK-) 小鼠胸腺激酶缺陷細(xì)胞株

Applications:transfection host (Roche FuGENE® Transfection Reagents)

Tumorigenic:Yes

Antigen Expression:H-2k

Cytogenetic Analysis:modal number = 45; range = 42 to 48.

The stemline chromosome number is hyperdiploid with the 2S component occurring at 3.8%. Twenty-one markers were common to nearly all S metaphases studied. Of these, 13 were two-armed chromosomes, and eight, acrocentric. Four markers were isochromosomes by Robertsonian translocations from chromosomes 4, 12, 14 and 15. Normal X, 12, 14, 15 and 16 were absent. The X chromosome was present as an arm of Rb(X.3). (See Cancer Genet. Cytogenet. 24: 95-107, 1987 for a detailed karyotype description.)



Age: 100 days

Gender: male

Comments:The cells are 5-bromo-2'-deoxyuridine resistant (BUDR, BrdUrd) and thymidine kinase (TK) deficient.

HAT sensitive.

Tested and found negative for ectromelia virus (mousepox).



Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Temperature: 37.0°C
Atmosphere: air, 95%; carbon dioxide (CO2), 5%


Subculturing: Protocol:
  1. Remove and discard culture medium.

  2. Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.

  3. Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).

    Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.


  4. Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting.

  5. Add appropriate aliquots of the cell suspension to new culture vessels.

  6. Incubate cultures at 37°C.


Subc*tion Ratio: A subc*tion ratio of 1:4 to 1:8 is recommended
Medium Renewal: Twice per week


Preservation: Freeze medium: complete growth medium, 95%; DMSO, 5%

Related Products:Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2002

recommended serum:ATCC 30-2020

parental cell line:ATCC CCL-1



References:868: Kit S, et al. Deletion of thymidine kinase activity from L cells resistant to bromodeoxyuridine. Exp. Cell Res. 31: 297-312, 1963. PubMed: 14056989

21410: Chen TR. Cytogenetics of somatic cell hybrids. II. Modal karyotypes and mosaicism in mouse mutant cell lines: LM(tk-), A9, and Rag. Cancer Genet. Cytogenet. 24: 95-107, 1987. PubMed: 3466673

25904: Hsu TC, Somers CE. Properties of L cells resistant to 5-bromodeoxyuridine. Exp. Cell Res. 26: 404-410, 1962. PubMed: 14449548

25907: Boone C, et al. Assignment of three human genes to chromosomes (LDH-A to 11, TK to 17, and IDH to 20) and evidence for translocation between human and mouse chromosomes in somatic cell hybrids (thymidine kinase-lactate dehydrogenase A-isocitrate dehydrogenase-C-11, E-17, and F-20 chromosomes). Proc. Natl. Acad. Sci. USA 69: 510-514, 1972. PubMed: 4110482

25910: Peterson JL, McBride OW. Cotransfer of linked eukaryotic genes and efficient transfer of hypoxanthine phosphoribosyltransferase by DNA-mediated gene transfer. Proc. Natl. Acad. Sci. USA 77: 1583-1587, 1980. PubMed: 6929511




















產(chǎn)品咨詢

留言框

  • 產(chǎn)品:

  • 您的單位:

  • 您的姓名:

  • 聯(lián)系電話:

  • 常用郵箱:

  • 省份:

  • 詳細(xì)地址:

  • 補(bǔ)充說(shuō)明:

  • 驗(yàn)證碼:

    請(qǐng)輸入計(jì)算結(jié)果(填寫(xiě)阿拉伯?dāng)?shù)字),如:三加四=7
聯(lián)系方式

郵箱:xiangfbio@163.com

地址:上海市虹口區(qū)四平路710號(hào)7層

咨詢熱線

400-821-8510

(周一至周日9:00- 19:00)

在線咨詢
  • 掃一掃 微信咨詢

Copyright©2025 上海復(fù)祥生物科技有限公司 All Right Reserved    備案號(hào):滬ICP備10013034號(hào)-2     sitemap.xml
技術(shù)支持:化工儀器網(wǎng)    管理登陸
国产a级毛片久久久精品毛片| 国产强伦姧在线观看| 在线播放免费人成毛片乱码| 狠狠亚洲婷婷综合色五月五月色 | 狠狠色噜噜狠狠狠狠97首创麻豆| 中国大陆高清aⅴ毛片| 国产精品免费视频| 欧美性猛交XXXX黑人| 中文字幕一区二区三区日韩精品| 欧美综合自拍亚洲综合图片区| 青青精品视频国产| 日本人妻巨大乳挤奶水免费| 亚洲国产精彩中文乱码av| 亚洲综合一区二区三区无码| 欧美日韩亚洲国产精品 | 国产av在线观看| 亚洲熟妇少妇任你躁在线观看无码 | 少妇熟女久久综合网色欲 | 成人夜色视频网站在线观看| 草草久久久无码国产专区| 亚洲成av人片一区二区密柚| YELLOW日本高清在线| 亚洲h在线播放在线观看h| 欧美三级a做爰在线观看| 一区二区三区内射美女毛片| 被群cao的合不拢腿h纯肉视频| 日产无码久久久久久精品| 欧美freese黑又粗又大| 亚洲一区二区三区偷拍女厕| 精品人妻无码一区二区三区不卡| 含着她的花蒂咬到高潮| 少妇无套内谢久久久久| 无码AⅤ精品一区二区三区浪潮| 久久综合狠狠综合久久综合88| 九月婷婷人人澡人人添人人爽| 久久人人爽人人片AV人成| 永久免费的啪啪网站免费观看浪潮| 国产露脸精品产三级国产av| 久久久久99精品成人片欧美| 香蕉欧美成人精品av在线| 又大又粗又爽免费视频a片|